Flow cytometry

Introduction Flow cytometry is a widely used method in biomedical research and increasingly also in clinical diagnostics. It is a powerful and rapid technique to analyze physical and chemical properties of single cells or particles as they are suspended in liquid and pass in a narrow line across laser beams. Fluorescence together with scattered laser light is then filtered, detected, and analyzed on the other side of the sample. In… READ MORE

Light-based enhancement of vaccination in humans

  Motivation Vaccines should activate all branches of immunity, including T cells and antibody responses, for generating a strong immune response. For therapeutic cancer vaccines, it is especially important to induce strong cytotoxic T-cell responses, since cytotoxic T-cells are the most potent immune cells for killing cancer cells. While cytotoxic T-cells can often be induced  with viral vector and nucleic acid-based vaccines, peptide-based vaccines often induce only weak cytotoxic T-cell… READ MORE

Optimizing photosensitizer administration for cancer therapy

  Motivation While Photofrin PDT is an approved oncological therapy for multiple indications, its wider use is hindered by prolonged skin photosensitivity lasting several weeks. The aim of this study is to investigate intratumoral Photofrin administration as a way to decrease skin photosensitivity compared to the standard IV injection protocol. Tumor necrosis after PDT was assessed with immunohistochemical staining and Photofrin distributions by fluorescence microscopy.       Results  … READ MORE

Optimized light delivery in pancreatic cancer

Motivation for the study Efficient light delivery to the whole tumor is one of the main aspects of phototherapy. The efficacy of phototherapy has been demonstrated for several cancers via superficial illumination. Interstitial illumination could enhance light delivery deeper inside the tumor and potentially result in more effective tumor eradication. Hence, the aim of this study is to compare if the therapeutic efficacy of phototherapy is dependent on the light… READ MORE

Light penetration depth in brain with different photosensitizers

  Motivation for the study Glioblastoma is the most aggressive and lethal brain cancer with an average prognosis of 15 months. Fluorescence-guided surgery (FGS) for glioblastoma was FDA-approved in 2017, while photodynamic therapy (PDT) remains an active area of clinical investigation with very promising results so far. The aim of PDT is to eradicate the invasive cancer cells within 2 cm of the resected area where the glioblastoma most often… READ MORE

Multimodal imaging microscope for tissue engineering applications

  Motivation for the study Tissue engineering is an evolving field of medicine that aims to grow new viable tissue for repairing or regenerating damaged tissues in the body. These tissue constructs are typically made by placing cells in support matrices that have proper growth-inducing factors. However, visualizing the development and composition of these tissue constructs is challenging with currently available optical imaging methods: they are either not capable of… READ MORE

Light delivery optimization for H&N cancer treatment

Results Illumination with one fiber Some level of tissue heating was observed at all used light intensities. At lower intensities (60-100 mW/cm), less than 5% of the tumor was subjected to >60 ⁰C temperature, a point where irreversible thermal damage will immediately occur (photothermal ablation). At intensities of 150-200 mW/cm, 15-20% of the tumor was at >60 ⁰C temperature. At 400 mW/cm, which is FDA-recommended light irradiance for iPDT, a… READ MORE

Antimicrobial PDT for prosthetic joint infections

Motivation for the study Periprosthetic joint infections after hip and knee replacement surgeries are associated with high patient morbidity and a large financial cost. Challenging two-stage revision surgeries are required with long antibiotics regimes that can have limited efficacy and cannot eradicate drug-resistant strains like MRSA (methicillin-resistant Staphylococcus aureus). This study aims to investigate the potential of photodynamic therapy with methylene blue to eradicate problematic bacteria associated with joint infections.… READ MORE

Photothermal therapy with platinum nanoparticles

Photothermal therapy efficiency Human ovarian cancer cells were plated on Petri dishes and incubated with platinum nanoparticles of varying sizes between 30 and 70 nm. After 24-hour incubation, irradiation was performed using a Modulight ML6600 laser at 808 nm together with an illumination kit (MLAKIT) tailored for Petri dish illumination. A small area of the dish was illuminated with the laser for five minutes with 45 W/cm2 irradiance. Calcein cell… READ MORE

Vision-sparing targeted phototherapy for uveal melanoma

Preliminary investigation of AU-011     Related Modulight products and Services   Related Publications Virus-Like Particle-Drug Conjugates Induce Protective, Long-lasting Adaptive Antitumor Immunity in the Absence of Specifically Targeted Tumor Antigens Rhonda C. Kines, Cynthia D. Thompson, Sean Spring, Zhenyu Li, Elisabet de los Pinos, Stephen Monks and John T. Schiller Cancer Immunology Research, 2021, 9 (6)   A Phase 1b/2 Open-label Clinical Trial to Evaluate the Safety and Efficacy… READ MORE