The goal was to investigate the effect of irradiance and light dose on a cancer cell line while keeping the photosensitive drug dosing constant. The well-by-well dose escalation plan was done in tabular format.
Cancer cell line incubated at 37°C in acidic culture
Photosensitive or photoactivated cancer drug (constant)
Variable dose of
100 and 600 mW/cm2
Experimental set up
Nexcelom Cellometer Auto2000 cell viability counter
The study of photosensitive or photoactivated drugs requires a controlled and systematic sample illumination process. Modulight ML8500 automated illumination system and ML7710 medical laser ensure the repeatable and accurate illumination of each sample. ML7710 supports up to 8 wavelengths allowing research of multiple drugs, or combining fluorescence measurements to the study plan.
Extracting and counting the cells with Cellometer Auto2000 (unit / ml)
Diluting the cells to 1 million / ml
Centrifuging and separating the cancer cells with cell stripper
Adding saline based new medium and serum
Mixing and incubating the sample
Washing the cells from free-floating drug
Confirming the drug bindings
Dispensing the cells to be radiated on 96-well sample plate
Illuminating the 96-well sample plate with Modulight ML8500 and ML7710
Incubating to eliminate close-to-death cells for appr. 1.5 h
ML8500 automated illumination system enables the researchers to investigate the effect of multiple independently variable parameters easily and reliably.
- Increase in dose (J/cm2) and in the overall cell death reached saturation at 50 J/cm2
- The efficacy of the drug was not dependent on the irradiance. 100 mW/cm2 and 600 mW/cm2 showed similar % cell death the same light dose.
- ML8500 automated illumination system enabled variation of 2 independent parameters (dose and irradiance) over 96-well plate in a single experiment.
|100 mW/cm2||600 mW/cm2|
Fully automated testing
Better validability and reliability
Optimized drug & light dose for the patient