Motivation for the study
Efficient light delivery to the whole tumor is one of the main aspects of phototherapy. The efficacy of phototherapy has been demonstrated for several cancers via superficial illumination. Interstitial illumination could enhance light delivery deeper inside the tumor and potentially result in more effective tumor eradication. Hence, the aim of this study is to compare if the therapeutic efficacy of phototherapy is dependent on the light delivery strategies.
A subcutaneous pancreatic cancer in vivo model was established by injecting BXPC3-GFP cancer cells into both flanks of the test subjects. Green fluorescent protein (GFP) serves as an indicator of the tumor cells. EFGR-targeted photoactive agent (Cetuximab-IR700DX) was administered intravenously 24 hours prior NIR laser irradiation. Light activation of the agent makes it phototoxic to the cells that have been bound to it. Only tumor on the right side received light (left side as a control) either using superficial illumination with a handpiece or interstitial illumination by inserting the optical fiber directly inside the tumor.
laser at 689 nm
Phototherapy (PT) with both superficial and interstitial illumination methods efficiently eradicated the tumor. However, significant tumor recurrence was observed 72 hours after phototherapy in case of superficial illumination, while recurrence was very limited with interstitial illumination. Damage to healthy surrounding tissues was not observed.
Graphs reproduced from the original publication with permission from the contact author of the publication.
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